We followed the instructions listed in the carolina transformation for ap biology. Also, it could be used to see what other genes can be turned on or off. Open the tubes and using a 100 l micropipette with a sterile tip, transfer 250 l of the ice cold transformation solution cacl 2. This web page was produced as an assignment for an ap biology course at montgomery. The plasmids are either pglo, puc1819 or puc1819 with a 6kb insert disrupting the lacz gene. Write a simple hypothesis by comparing your sample with that of a partners, or design what to sample and test. Since 1989, there have been 12 laboratorieslovingly nicknamed the dirty dozenthat are suggested curricula for ap biology classes. This lab demonstrates how bacteria can become antibiotic resistant. The progression from dna rna protein trait is shown with the bacteria e.
Information for teacherssafety instructions although the e. Its going to be my first year teaching ap bio, so your videos are very. For ap biology, your participation in lab is an integral part of the class experience as well as your grade 40%. If the ampicillin resistant dna is introduced into the e. Bacterial transformation setup tuesday complete bacterial transformation lab. Introduction abstract our alternate hypothesis for this experiment will be that the pglo dna will incorporate in the e. This gene transfer is accomplished with the aid of a plasmid, a naturallyoccuring small piece of circular dna in bacteria. Next generation science standards and the newest ap biology materials support. Classic bacterial transformation lab using pglo plasmid from biorad. Bacterial transformation lab ap biology heather swain dp. Prepare your students for medical and lab tech careers with carolina s wide range of. Students transform bacterial cells with a novel gene that.
View lab report 3867838apbiologytransformationlabreport from microbiolo at national university of singapore. The art of bacterial transformation introduction purpose. One method to achieve this is through chemical competence with heat shock. Collectively, they created ap biology investigative labs. Bacterial transformation lab report laura downing dp. Introduction bacteria are microscopic, singlecelled organisms.
The pglo bacterial transformation lab is based off the molecular biology ap biology lab involving transformation. Wednesday ap biology lab 9 transpiration formal report due on april 8 thursday quiz on plants, begin lecture on immune system, using personal notes, not book friday complete lecture and notes on immune system. In molecular biology, transformation refers to a form of genetic exchange in. Mar 26, 2015 classic bacterial transformation lab using pglo plasmid from biorad. Some bacteria have another method of transferring dna and producing recombinants that does not require conjugation. Dna on plate 1 since there is no antibiotic present the wildtype bacteria grew normally and formed a lawn across the whole plate. They have the capacity to double every twenty minutes and make a favorable carrier of recombinant dna. Stay connected to your students with prezi video, now in microsoft teams. Ap biology bacterial transformation lab by kenneth. Education center k12 lessons and laboratories classroom activities in plant biotechnology. In this investigation, students will first acquire the tools to transform e. Bacterial transformation lab report, sample of homeworks. To study the behavior of escherichia coli once it has been introduced to a foreign gene. Genetic transformation is a process by which the genetic material carried by an individual cell is altered by the incorporation of foreign exogenous dna into its genome medicinenet, 1999.
Answers are provided in the instructors answer guide. Ap bio pglo transformation formal lab report, sample of. The plasmid pglo contains an antibioticresistance gene, ampr, and the gfp gene is regulated by the control region of the ara operon. Since only some of the cells exposed to the amp r plasmids will actually take them in, only some cells will be transformed. Our results supported our hypothesis and the transformation was successful. Bacterial transformation lab report free essay example. Biology is brought to you with support from the our mission is to provide a free, worldclass education to anyone, anywhere. Ap bio pglo transformation formal lab report writework. The answers will vary but should be in the range of 103 104 transformantsg dna. The pglo plasmid contained a gene for the green fluorescent protein gfp from a bioluminescent jellyfish and a gene for resistance to ampicillin, an antibiotic. The goal of the lab was to identify the plasmids because it was unknown which plasmids contained which genes. Thursday quiz on plants, begin lecture on immune system, using personal notes, not book friday complete lecture and notes on immune system. Choose from our kits, follow a college board lab, or design your own with our wide variety of equipment and supplies. Bacterial transformation is of central importance in molecular biology.
Select lab 6 molecular biology part i transformation in bacteria 3. Describe plasmid uptake and how transformation is determined 6 points maximum. Transformation lab a plasmid discovery labratory 6, ap biology abstract. Duplication of any part of this document is permitted for classroom use only. This report analyzes an experiment conducted in order to transform e. Concepts you will need to know include identifying a word to describe. He explains how you can use the pglo plasmid to produce glowing e. In the transformation lab designed by the carolina biological supply co. Genetic transformation is an active uptake of free dna by a bacterial cell like e.
Pearson, as an active contributor to the biology learning community, is pleased to provide free access to the classic edition of the biology place to all educators and their students. They span the globe in their topics and their techniques. More specifically, a previously prepared pglo plasmidwhich consisted of the gene to be clonedwas used to transform nonpathogenic bacteria. Even though we referenced the diagram in our lab manual that showed how much. Thus you will see only individual colonies on the plate. We have kits for new and traditional ap biology labs. Bacterial transformation some bacteria have another method of transferring dna and producing recombinants that does not require conjugation. All in all, the lab was fun and a success in transforming the bacteria, ecoli, into one that is antibiotic resistant and into one that can glow. In the transformation lab, we discovered the process of bacterial genetic transformation and how to calculate transformation efficiency. Write a hypothesis that reflects the experiment and predict experi. Bacterial transformation is what happens when a bacterial cell accepts outside dna and incorporates it into its own dna, something that generally happens with plasmids. Oct 16, 2012 this video demonstrates how to perform a bacterial transformation using the biorad pglo bacterial transformation kit. View lab report 3867838 ap biology transformation lab report from microbiolo at national university of singapore. Biology is brought to you with support from the amgen foundation.
Only transformed cells can grow on agar with ampicillin. Prepare your students for medical and lab tech careers with carolina s wide range of equipment, kits and models. Connect concepts with techniques and put them into context with. Coli bacterial cells through the transformation process carolina biological supply co. Ap biologyinvestigation 8bacterial transformation lab. Conceptual approaches to biology for majors i bio 281. Ap bio pglo transformation formal lab report, sample of essays. Cold spring harbor laboratorys dna learning center presents this course as a service to help engage teachers and students in china during. The labs in this manual support the concepts, content, and science practices within the revised ap biology course. We enable science by offering product choice, services, process excellence and our people make it happen. Big genetics and information transfer 3 ap central. The pglolb control plate can be compared to any of the lbamp plates to show that plasmid uptake is required for the growth in the presence. Topic description 1 point each transformation defined process of transformation of a plasmid isolation isolate plasmidsagar plate that grows only colonies of resistance gene antibiotic use of antibiotic resistancesensitivity genes. For more information, visit this video demonstrates how to perform a bacterial.
Learn vocabulary, terms, and more with flashcards, games, and other study tools. Bacterial transformation lab ap biology during this bacterial transformation lab, we inserted a plasmid containing a antibioticresistance gene. Their genetic information is encoded in one large chromosome in addition to plasmids, which are small, circular molecules of dna campbell and reece 2005. Transformation lab report biol 1100 csula studocu bacterial transformation the pglo system catalog number 1660003edu. Bacterial transformation advanced student laboratory kit. Transformation was discovered in streptococcus pneumoniae in 1928 by frederick griffith. Escherichia coli are commensal gram negative bacteria found in the guts of humans. Open the tubes and using a 100 l micropipette with a sterile tip, transfer 250 l of the ice cold transformation solution cacl. The conversion of one genotype into another by the introduction of exogenous dna that is, bits of dna from an external source is termed transformation. See science manual bacterial transformation lab for complete list of materials and procedures. For technical service call your local biorad office or in the u. During this bacterial transformation lab, we inserted a plasmid containing a.
Bacterial transformation, the process in which a plasmid is induced into a bacterial host, is one example of genetic engineering, which is any humancreated changes in an organisms dna. See more ideas about bacterial transformation, ap biology and microbiology. Investigation 8 bacterial plasmidbased genetic transformation, enables students to manipulate genetic information in a. Answer the lab packet questions as you proceed through the virtual lab. The purpose of this lab was to discover whether or not one could. Read the lab safety information and write the complete procedure pp. This comparison shows that genetic transformation produces bacterial colonies that can grow on ampicillin due to the uptake of the pglo plasmid and the expression of the ampicillin resistance gene.
Working in teams, each team uses an unidentified plasmid that is either kanamycinresistant or ampicillinresistant and could possibly also code for the gene for green fluorescent protein gfp. New plasmid engineering and bacterial transformation highly motivated ap biology and research classes only students must have completed living environment this is a rigorous laboratory experience suitable for highly motivated students in ap biology or a research class with strong, independent laboratory skills. Transforming li strains with green fluorescent protein. Bacterial transformation an introduction to genetic. Transformation defined process of transformation of a plasmid isolation isolate plasmidsagar plate that grows only colonies of resistance gene antibiotic use of antibiotic resistancesensitivity genes detailed description of antibiotic resistance lab procedure gel electrophoresis isolate. In lab groups, we genetically engineered the bacteria with pglo plasmids containing specific codes for gfp green fluorescent protein and ampicillin, an antibiotic, resistance. Address and record the following in your laboratory notebook or on a. Dec 17, 2014 transformation lab a plasmid discovery labratory 6, ap biology abstract. Ravesiweinstein science coordinator attleboro high school 100 rathbun willard dr. The purpose of this lab is to insert genes that will make e. The lbamp pglo plate will have no growth, the lb pglo plate will have a lawn of growth meaning colonies covering. Bacterial transformation lab by lindsay young on prezi. Bacterial transformation lab report bio 281 asu studocu. When lab is complete, collect all petri dishes, open, and immerse in a 10%.
Nathan smith abstract bacterial transformation is the easiest form because bacteria is a singlecelled organism, therefore happens faster and has a higher success rate of genetic transformation. This video demonstrates how to perform a bacterial transformation using the biorad pglo bacterial transformation kit. In the bacterial transformation advanced inquiry lab kit for ap biology, study biotechnology, which raises a lot of questions about the ethics of modifying. Ap biology investigation 8 bacterial transformation lab walkthrough. Essentially, genetic transformation involves the process where a cell in this lab, a bacterial cell takes up foreign dna from its surroundings and incorporates it into its own dna.
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